Ballesteros, Isabel , Maria P. Martin & Javier Dieguez-Uribeondo. First isolation of Aphanomyces frigidophilus (Saprolegniales) in Europe. Mycotaxon 95: 335 - 340. 2006.

ABSTRACT: Aphanomyces frigidophilus was isolated and described for the first time in Europe. The isolate was characterized by studying its ability to undergo repeated zoospore emergence, to parasitize crayfish, and to produce chitinase constitutively, and by sequencing the internal transcribed spacer of nuclear ribosomal DNA (ITS1+5.8S+ITS2). The physiological properties studied differed from those of the "crayfish plague" parasite, A. astaci, but they were similar to those of saprobiotic Aphanomyces species. The ITS nrDNA sequence obtained from this isolate corresponded to A. frigidophilus. 

KEYWORDS: Aphanomyces astaci, crayfish, conservation, rDNA, taxonomy