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Whiting, Elizabeth, Marcos Cunha & W. Douglas Gubler. Phaeomoniella chlamydospora and Phaeoacremonium species distinguished through cultural characters and ribosomal DNA sequence analysis. Mycotaxon 92: 351-360. 2005.

ABSTRACT: Representative isolates of Phaeomoniella chlamydospora and Phaeoacremonium spp. associated with Petri grapevine decline were analyzed using partial sequences of the ITS region of nuclear ribosomal DNA (nrDNA), growth at 35?C, and ability to oxidize gallic and tannic acids. Cultural, physiological, and molecular analyses support separation of Phaeomoniella chlamydospora from Phaeoacremonium spp. and support separation of Phaeoacremonium inflatipes from other Phaeoacremonium spp. Two closely related species, P. aleophilum and P. angustius, clustered in a single clade based on DNA sequence data, but formed two groups based on cultural and physiological tests. A dichotomous key is presented that can be used to distinguish between Phaeomoniella chlamydospora and Phaeoacremonium spp.

KEYWORDS: hyphomycetes, DNA analysis, organic acids

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