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Sharifnabi, Bahram, Neena Mitter & D. K. Agarwal. Characterization of Ustilago tritici and U. nuda on wheat and barley. II. RAPD analysis. Mycotaxon 86: 87 - 97. 2003.

ABSTRACT: Monokaryotic haploid hyphae of fourteen isolates of Ustilago spp. pathogenic on wheat and barley were analyzed by random amplified polymorphic DNA analysis (RAPD) using twenty-five decamer primers. The results of the RAPD analyses showed that a high degree of variability exists among isolates of Ustilago tritici. Isolate Ut12 gave a distinct pattern and was separated from all others by UPGMA cluster analysis. Amplification products obtained with primers OPN5 and OPM6 were able to clearly separate U. nuda isolates from all the U. tritici isolates. The study highlights the fact that DNA polymorphism can be used to separate U. nuda from U. tritici, which could not be done on the basis of teliospore morphology.

KEYWORDS: Monokaryotic haploid hyphae, Loose smut, DNA analysis, UPGMA cluster analysis



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