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Liu, Xiao-yong, He Huang & Ru-yong Zheng. Relationships within Cunninghamella based on sequence analysis of ITS rDNA. Mycotaxon 80: 77-95. 2001.

ABSTRACT: The internal transcribed spacers of ribosomal DNA (rDNA ITS) of 20 strains representing 12 species of the genus Cunninghamella were amplified using the polymerase chain reacton (PCR) with a pair of oligonucleotide primers ITS1 and ITS4. The PCR products were cloned and sequenced. The entire lengths of the rDNA ITS region of the 20 strains range from 611 to 844 bp with great differences among species. Generally, sequence analyses provide different values at the strain, variety and species level: genetic distances among strains and varieties of the same species and among different species are respectively less than 0.020, 0.024-0.059, and more than 0.102. In a consensus tree inferred by the neighbor-joining method, although C. septata clustered with the 4 varieties of C. echinulata in the same clade, and C. phaeospora and C. multiverticillata formed a clade, they are resolved as systematically distinct species. Results of the other taxa in the present sequence analysis are consistent with morphological studies that support our taxonomic treatments of the genus: all different strains or different varieties of the same species clustered in the same clade, while different species clustered in different clades.



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