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Platas, G., V. Gonzaléz, J. Collado, J. Martín, C. Montes & F. Peláez. Ascription of poorly defined taxa to taxonomic entities using molecular phylogenies: a case study on Nodulisporium sp. producers of nodulisporic acid. Mycotaxon 109: 443–460. 2009.

ABSTRACT:  DNA sequences of the ITS1-5.8S-ITS2 region, b-tubulin, and a-actin genes were obtained from a set of Nodulisporium sp. isolates producing the potent anti-parasitic agent, nodulisporic acid, and other related strains. These sequences were used to confirm the molecular homogeneity of these NA-producers, to determine their most closely related taxa within genus Hypoxylon, and to explore the phylogenetic relationships between these strains and other hypoxyloid sequences from public DNA databases. The results from all the loci analyzed show that this group of strains constitutes a monophyletic taxon. This taxon would be included within a cluster containing endophytic, tropical xylariaceous isolates lacking defined teleomorph and taxonomic status, together with several Hypoxylon species such as H. investiens, H. kanchanapisekii, and H. anthochroum.

KEYWORDS:  phylogeny, Xylariales, xylariaceous

   
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